PKA R2 (PRKAR2A) Mouse Monoclonal Antibody [Clone ID: OTI1B4]

Specifications

Product Data
Clone Name	OTI1B4
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:50, IF 1:100, Flow: 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human PRKAR2A (NP_004148) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	45.3 kDa
Gene Name	protein kinase cAMP-dependent type II regulatory subunit alpha
Database Link	NP_004148 Entrez Gene 19087 MouseEntrez Gene 29699 RatEntrez Gene 5576 Human UniProt ID P13861
Background	cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
Synonyms	PKR2; PRKAR2
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Apoptosis, Insulin signaling pathway

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