UAP56 (DDX39B) Mouse Monoclonal Antibody [Clone ID: OTI2E4]

Specifications

Product Data
Clone Name	OTI2E4
Applications	IF, WB
Recommend Dilution	WB 1:500, IF 1:100
Reactivity	Human, Dog, Rat, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human BAT1(NP_542165) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	48.8 kDa
Gene Name	DExD-box helicase 39B
Database Link	NP_542165 Entrez Gene 53817 MouseEntrez Gene 114612 RatEntrez Gene 474839 DogEntrez Gene 7919 Human UniProt ID Q13838
Background	This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and it also plays an important role in mRNA export from the nucleus to the cytoplasm. This gene belongs to a cluster of genes localized in the vicinity of the genes encoding tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on both chromosomes 6 and 11. Read-through transcription also occurs between this gene and the upstream ATP6V1G2 (ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2) gene. [provided by RefSeq, Feb 2011]
Synonyms	BAT1; D6S81E; UAP56
Reference Data
Protein Pathways	Spliceosome

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