Profilin 1 (PFN1) Mouse Monoclonal Antibody [Clone ID: OTI2D2]

Specifications

Product Data
Clone Name	OTI2D2
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
Reactivity	Human, Dog, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human PFN1 (NP_005013) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	14.9 kDa
Gene Name	profilin 1
Database Link	NP_005013 Entrez Gene 18643 MouseEntrez Gene 64303 RatEntrez Gene 607397 DogEntrez Gene 710753 MonkeyEntrez Gene 5216 Human UniProt ID P07737
Background	The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome. [provided by RefSeq]
Synonyms	ALS18
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Regulation of actin cytoskeleton

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