elF2 alpha (EIF2S1) Mouse Monoclonal Antibody [Clone ID: OTI5D10]

Specifications

Product Data
Clone Name	OTI5D10
Applications	IF, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:50, IF 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	35.9 kDa
Gene Name	eukaryotic translation initiation factor 2 subunit alpha
Database Link	NP_004085 Entrez Gene 13665 MouseEntrez Gene 54318 RatEntrez Gene 1965 Human UniProt ID P05198
Background	The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]). [supplied by OMIM]
Synonyms	EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A
Reference Data

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