RASSF1 Mouse Monoclonal Antibody [Clone ID: OTI2B11]

Specifications

Product Data
Clone Name	OTI2B11
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human RASSF1 (NP_009113) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	38.6 kDa
Gene Name	Ras association domain family member 1
Database Link	NP_009113 Entrez Gene 56289 MouseEntrez Gene 363140 RatEntrez Gene 11186 Human UniProt ID Q9NS23
Background	This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Synonyms	123F2; NORE2A; RASSF1A; RDA32; REH3P21
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Bladder cancer, Non-small cell lung cancer, Pathways in cancer

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