GBA3 Mouse Monoclonal Antibody [Clone ID: OTI3E8]

Specifications

Product Data
Clone Name	OTI3E8
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-150 and 370-469 of human GBA3(NP_066024) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	53.5 kDa
Gene Name	glucosylceramidase beta 3 (gene/pseudogene)
Database Link	NP_066024 Entrez Gene 57733 Human UniProt ID Q9H227
Background	GBA3, or cytosolic beta-glucosidase (EC 3.2.1.21), is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (de Graaf et al., 2001 [PubMed 11389701]). GBA3 also has significant neutral glycosylceramidase activity (EC 3.2.1.62), suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (Hayashi et al., 2007 [PubMed 17595169]). [supplied by OMIM]
Synonyms	CBG; CBGL1; GLUC; KLRP
Reference Data
Protein Pathways	Cyanoamino acid metabolism, Starch and sucrose metabolism

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