LCMT1 Mouse Monoclonal Antibody [Clone ID: OTI1G5]

Specifications

Product Data
Clone Name	OTI1G5
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human LCMT1(NP_057393) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	38.2 kDa
Gene Name	leucine carboxyl methyltransferase 1
Database Link	NP_057393 Entrez Gene 30949 MouseEntrez Gene 361643 RatEntrez Gene 51451 Human UniProt ID Q9UIC8
Background	LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]). [supplied by OMIM, Mar 2008]
Synonyms	CGI-68; LCMT; PPMT1
Reference Data
Protein Pathways	Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism

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