LGR5 Mouse Monoclonal Antibody [Clone ID: OTI2A2]

Specifications

Product Data
Clone Name	OTI2A2
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100, IP 2ug/500ul
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 250-550 of human LGR5 (NP_003658) produced in HEK293T Cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	99.8 kDa
Database Link	NP_003658 Entrez Gene 14160 MouseEntrez Gene 299802 Rat
Synonyms	FEX; GPR49; GPR67; GRP49; HG38
Reference Data
Protein Families	Druggable Genome, GPCR, Transmembrane

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