Adiponectin (ADIPOQ) Mouse Monoclonal Antibody [Clone ID: OTI1H1]

Specifications

Product Data
Clone Name	OTI1H1
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 19-244 of human ADIPOQ(NP_004788) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	24.9 kDa
Gene Name	adiponectin, C1Q and collagen domain containing
Database Link	NP_004788 Entrez Gene 9370 Human UniProt ID Q15848
Background	This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]
Synonyms	ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Adipocytokine signaling pathway, PPAR signaling pathway, Type II diabetes mellitus

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