RUVBL2 Mouse Monoclonal Antibody [Clone ID: OTI1H10]

Specifications

Product Data
Clone Name	OTI1H10
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 113-370 of human RUVBL2(NP_006657) prduced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	51 kDa
Gene Name	RuvB like AAA ATPase 2
Database Link	NP_006657 Entrez Gene 20174 MouseEntrez Gene 292907 RatEntrez Gene 10856 Human UniProt ID Q9Y230
Background	This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq]
Synonyms	CGI-46; ECP51; INO80J; REPTIN; RVB2; TIH2; TIP48; TIP49B
Reference Data
Protein Families	Transcription Factors

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