Epsin 2 (EPN2) Mouse Monoclonal Antibody [Clone ID: OTI1G3]

Specifications

Product Data
Clone Name	OTI1G3
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human, Monkey, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human EPN2(NP_055779) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	68.3 kDa
Gene Name	epsin 2
Database Link	NP_055779 Entrez Gene 13855 MouseEntrez Gene 60443 RatEntrez Gene 702600 MonkeyEntrez Gene 22905 Human UniProt ID O95208
Background	This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
Synonyms	EHB21
Reference Data
Protein Pathways	Endocytosis

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