GADD34 (PPP1R15A) Mouse Monoclonal Antibody [Clone ID: OTI3D12]

Specifications

Product Data
Clone Name	OTI3D12
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human, Dog, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human PPP1R15A(NP_055145) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	73.3 kDa
Gene Name	protein phosphatase 1 regulatory subunit 15A
Database Link	NP_055145 Entrez Gene 17872 MouseEntrez Gene 171071 RatEntrez Gene 476419 DogEntrez Gene 718350 MonkeyEntrez Gene 23645 Human UniProt ID O75807
Background	This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008]
Synonyms	GADD34
Reference Data
Protein Families	Druggable Genome

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