CD31 (PECAM1) Mouse Monoclonal Antibody [Clone ID: OTI1C2]

Specifications

Product Data
Clone Name	OTI1C2
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:200 - 1:1000, IHC 1:150, FLOW 1:50
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human PECAM1(NP_000433) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	82.4 kDa
Gene Name	platelet and endothelial cell adhesion molecule 1
Database Link	NP_000433 Entrez Gene 5175 Human UniProt ID P16284
Background	The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May
Synonyms	CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs), Leukocyte transendothelial migration

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