p15 INK4b (CDKN2B) Mouse Monoclonal Antibody [Clone ID: OTI8H4]

Specifications

Product Data
Clone Name	OTI8H4
Applications	IF, IHC, WB
Recommend Dilution	WB 1:500~2000, IHC 1:150, IF 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human CDKN2B(NP_004927) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	14.5 kDa
Gene Name	cyclin dependent kinase inhibitor 2B
Database Link	NP_004927 Entrez Gene 12579 MouseEntrez Gene 25164 RatEntrez Gene 1030 Human UniProt ID P42772
Background	This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq]
Synonyms	CDK4I; INK4B; MTS2; P15; p15INK4b; TP15
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway

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