MEK3 (MAP2K3) Mouse Monoclonal Antibody [Clone ID: OTI3C8]

Specifications

Product Data
Clone Name	OTI3C8
Applications	IF, IHC, WB
Recommend Dilution	WB 1:200~2000, IHC 1:150, IF 1:100
Reactivity	Human, Dog, Rat, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinan protein of human MAP2K3(NP_659731) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	39.1 kDa
Gene Name	mitogen-activated protein kinase kinase 3
Database Link	NP_659731 Entrez Gene 26397 MouseEntrez Gene 303200 RatEntrez Gene 489547 DogEntrez Gene 5606 Human UniProt ID P46734
Background	The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]
Synonyms	MAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Transcription Factors
Protein Pathways	Amyotrophic lateral sclerosis (ALS), Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway

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