VPS28 Mouse Monoclonal Antibody [Clone ID: OTI1A8]

Specifications

Product Data
Clone Name	OTI1A8
Applications	IF, IHC, WB
Recommend Dilution	WB 1:200~2000, IHC 1:150, IF 1:100
Reactivity	Human, Dog, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human VPS28(NP_057292) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	25.2 kDa
Gene Name	VPS28 subunit of ESCRT-I
Database Link	NP_057292 Entrez Gene 66914 MouseEntrez Gene 300052 RatEntrez Gene 607677 DogEntrez Gene 702412 MonkeyEntrez Gene 51160 Human UniProt ID Q9UK41
Background	This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally. [provided by RefSeq, Jul 2008]
Synonyms	MGC60323
Reference Data
Protein Pathways	Endocytosis

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