EPCAM Mouse Monoclonal Antibody [Clone ID: OTI2B1]

Specifications

Product Data
Clone Name	OTI2B1
Applications	FC, IF, IHC, WB
Recommend Dilution	WB: 1:1000-1:4000, IF 1:100, Flow 1:100, IHC: 1:150-1:500
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human EPCAM(NP_002345) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	32.7 kDa
Gene Name	epithelial cell adhesion molecule
Database Link	NP_002345 Entrez Gene 4072 Human UniProt ID P16422
Background	This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Synonyms	DIAR5; EGP-2; EGP40; EGP314; ESA; HNPCC8; KS1/4; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1
Reference Data
Protein Families	ES Cell Differentiation/IPS, Transmembrane

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