TMS1 (PYCARD) Mouse Monoclonal Antibody [Clone ID: OTI1A2]

Specifications

Product Data
Clone Name	OTI1A2
Applications	FC, WB
Recommend Dilution	WB 1:500~2000, FLOW 1:100
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human PYCARD (NP_037390) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	21.4 kDa
Gene Name	PYD and CARD domain containing
Database Link	NP_037390 Entrez Gene 29108 Human UniProt ID Q9ULZ3
Background	This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Synonyms	ASC; CARD5; TMS; TMS-1; TMS1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway

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