NM23A (NME1) Mouse Monoclonal Antibody [Clone ID: OTI4H2]

Specifications

Product Data
Clone Name	OTI4H2
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human NME1 (NP_937818) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	19.5 kDa
Gene Name	NME/NM23 nucleoside diphosphate kinase 1
Database Link	NP_937818 Entrez Gene 18102 MouseEntrez Gene 191575 RatEntrez Gene 4830 Human UniProt ID P15531
Background	This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]
Synonyms	AWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Metabolic pathways, Purine metabolism, Pyrimidine metabolism

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