TWIST2 Mouse Monoclonal Antibody [Clone ID: OTI9F11]

Specifications

Product Data
Clone Name	OTI9F11
Applications	WB
Recommend Dilution	WB 1:2000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human TWIST2 (NP_476527) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	17.9 kDa
Gene Name	twist family bHLH transcription factor 2
Database Link	NP_476527 Entrez Gene 13345 MouseEntrez Gene 59327 RatEntrez Gene 117581 Human UniProt ID Q8WVJ9
Background	Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Twist. It is thought that during osteoblast development this protein may inhibit osteoblast maturation and maintain cells in a preosteoblast phenotype. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2012]
Synonyms	AMS; BBRSAY; bHLHa39; DERMO1; FFDD3; SETLSS
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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