Carboxypeptidase M (CPM) Mouse Monoclonal Antibody [Clone ID: OTI8E2]

Specifications

Product Data
Clone Name	OTI8E2
Applications	IHC, WB
Recommend Dilution	WB 1:500, IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 124-423 of human CPM(NP_001865) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	48.6 kDa
Gene Name	carboxypeptidase M
Database Link	NP_001865 Entrez Gene 70574 MouseEntrez Gene 314855 RatEntrez Gene 1368 Human UniProt ID P14384
Background	The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2008]
Synonyms	carboxypeptidase M
Reference Data
Protein Families	Druggable Genome, Protease

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