Islet 1 (ISL1) Mouse Monoclonal Antibody [Clone ID: OTI2D8]

Specifications

Product Data
Clone Name	OTI2D8
Applications	WB
Recommend Dilution	WB 1:500
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 134-349 of human ISL1(NP_002193) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	38.9 kDa
Gene Name	ISL LIM homeobox 1
Database Link	NP_002193 Entrez Gene 16392 MouseEntrez Gene 64444 RatEntrez Gene 3670 Human UniProt ID P61371
Background	This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008]
Synonyms	Isl-1; ISLET1
Reference Data
Protein Families	ES Cell Differentiation/IPS, Transcription Factors

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