NDUFB11 Mouse Monoclonal Antibody [Clone ID: OTI2E10]

Specifications

Product Data
Clone Name	OTI2E10
Applications	WB
Recommend Dilution	WB 1:2000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 30-163 of human NDUFB11(NP_061929) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	18.2 kDa
Gene Name	NADH:ubiquinone oxidoreductase subunit B11
Database Link	NP_061929 Entrez Gene 54539 Human UniProt ID Q9NX14
Background	NDUFB11 is a component of mitochondrial complex I. Complex I catalyzes the first step in the electron transport chain, the transfer of 2 electrons from NADH to ubiquinone, coupled to the translocation of 4 protons across the membrane (Carroll et al., 2002 [PubMed 12381726]). [supplied by OMIM, Feb 2009]. Transcript Variant: This variant (2) uses a different splice site in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) has a shorter C-terminus when it is compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AY359056.1, BE781599.1 [ECO:0000332] RNAseq introns :: single sample supports all introns ERS025084, ERS025087 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##
Synonyms	CI-ESSS; ESSS; Np15; NP17.3; P17.3
Reference Data
Protein Families	Transmembrane

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