ERCC8 Mouse Monoclonal Antibody [Clone ID: OTI2G1]

Specifications

Product Data
Clone Name	OTI2G1
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human ERCC8(NP_001007235) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	43.9 kDa
Gene Name	ERCC excision repair 8, CSA ubiquitin ligase complex subunit
Database Link	NP_001007235 Entrez Gene 71991 MouseEntrez Gene 310071 RatEntrez Gene 1161 Human UniProt ID Q13216
Background	This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]
Synonyms	CKN1; CSA; UVSS2
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Nucleotide excision repair, Ubiquitin mediated proteolysis

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