Estrogen Receptor 1 (ESR1) Mouse Monoclonal Antibody [Clone ID: OTI5E11]

Specifications

Product Data
Clone Name	OTI5E11
Applications	IHC, WB
Recommend Dilution	WB 1:200~2000, IHC 1:150
Reactivity	Human, Dog, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-310 of human ESR1 (NP_000116) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	66 kDa
Gene Name	estrogen receptor 1
Database Link	NP_000116 Entrez Gene 13982 MouseEntrez Gene 24890 RatEntrez Gene 403640 DogEntrez Gene 2099 Human UniProt ID P03372
Background	This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]
Synonyms	ER; Era; ESR; ESRA; ESTRR; NR3A1
Reference Data
Protein Families	Druggable Genome, Nuclear Hormone Receptor, Transcription Factors

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