GALNT10 Mouse Monoclonal Antibody [Clone ID: OTI2A12]

Specifications

Product Data
Clone Name	OTI2A12
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:500
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-318 of human GALNT10(NP_938080) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	68.8 kDa
Gene Name	polypeptide N-acetylgalactosaminyltransferase 10
Database Link	NP_938080 Entrez Gene 170501 RatEntrez Gene 171212 MouseEntrez Gene 55568 Human UniProt ID Q86SR1
Background	This gene encodes a member of the GalNAc polypeptide N-acetylgalactosaminyltransferases. These enzymes catalyze the first step in the synthesis of mucin-type oligosaccharides. These proteins transfer GalNAc from UDP-GalNAc to either serine or threonine residues of polypeptide acceptors. The protein encoded by this locus may have increased catalytic activity toward glycosylated peptides compared to activity toward non-glycosylated peptides. [provided by RefSeq, Apr 2010]
Synonyms	GALNACT10; PPGALNACT10; PPGANTASE10
Reference Data
Protein Families	Transmembrane
Protein Pathways	Metabolic pathways, O-Glycan biosynthesis

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