Clathrin light chain (CLTA) Mouse Monoclonal Antibody [Clone ID: OTI2C2]

Specifications

Product Data
Clone Name	OTI2C2
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:500
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human CLTA(NP_001824) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	23.5 kDa
Gene Name	clathrin light chain A
Database Link	NP_001824 Entrez Gene 12757 MouseEntrez Gene 83800 RatEntrez Gene 1211 Human UniProt ID P09496
Background	Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq, May 2010]
Synonyms	LCA
Reference Data
Protein Pathways	Endocytosis, Huntington's disease, Lysosome

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