IQGAP1 Mouse Monoclonal Antibody [Clone ID: OTI7F5]

Specifications

Product Data
Clone Name	OTI7F5
Applications	FC, IHC, WB
Recommend Dilution	WB 1:500~2000, IHC 1:5000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 464-609 of human IQGAP1(NP_003861) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	189.1 kDa
Gene Name	IQ motif containing GTPase activating protein 1
Database Link	NP_003861 Entrez Gene 29875 MouseEntrez Gene 361598 RatEntrez Gene 8826 Human UniProt ID P46940
Background	This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008]
Synonyms	HUMORFA01; p195; SAR1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Adherens junction, Regulation of actin cytoskeleton

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