PYCR1 Mouse Monoclonal Antibody [Clone ID: OTI4F2]

Specifications

Product Data
Clone Name	OTI4F2
Applications	IHC, WB
Recommend Dilution	WB 1:500~2000, IHC 1:500
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-319 of human PYCR1 (NP_008838) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	33.2 kDa
Gene Name	pyrroline-5-carboxylate reductase 1
Database Link	NP_008838 Entrez Gene 209027 MouseEntrez Gene 287877 RatEntrez Gene 5831 Human UniProt ID P32322
Background	This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Synonyms	ARCL2B; ARCL3B; P5C; P5CR; PIG45; PP222; PRO3; PYCR
Reference Data
Protein Pathways	Arginine and proline metabolism, Metabolic pathways

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