UBE1C (UBA3) Mouse Monoclonal Antibody [Clone ID: OTI1B10]

Specifications

Product Data
Clone Name	OTI1B10
Applications	WB
Recommend Dilution	WB 1:500
Reactivity	Human, Dog, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-463 of human UBA3 (NP_003959) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	51.7 kDa
Gene Name	ubiquitin like modifier activating enzyme 3
Database Link	NP_003959 Entrez Gene 22200 MouseEntrez Gene 117553 RatEntrez Gene 476560 DogEntrez Gene 696493 MonkeyEntrez Gene 9039 Human UniProt ID Q8TBC4
Background	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Synonyms	hUBA3; NAE2; UBE1C
Reference Data
Protein Pathways	Ubiquitin mediated proteolysis

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