MTHFR Mouse Monoclonal Antibody [Clone ID: OTI2H3]

Specifications

Product Data
Clone Name	OTI2H3
Applications	WB
Recommend Dilution	WB 1:250~500
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-230 of human MTHFR (NP_005948) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	74.4 kDa
Gene Name	methylenetetrahydrofolate reductase
Database Link	NP_005948 Entrez Gene 17769 MouseEntrez Gene 362657 RatEntrez Gene 4524 Human UniProt ID P42898
Background	The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency. [provided by RefSeq, Oct 2009]
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Methane metabolism, One carbon pool by folate

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