UAP56 (DDX39B) Mouse Monoclonal Antibody [Clone ID: OTI8B8]

Specifications

Product Data
Clone Name	OTI8B8
Applications	IHC, WB
Recommend Dilution	WB 1:500, IHC 1:500
Reactivity	Human, Dog, Monkey, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 319-428 of human DDX39B (NP_004631) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	48.8 kDa
Gene Name	DExD-box helicase 39B
Database Link	NP_004631 Entrez Gene 53817 MouseEntrez Gene 114612 RatEntrez Gene 474839 DogEntrez Gene 715233 MonkeyEntrez Gene 7919 Human UniProt ID Q13838
Background	Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2; the effect of ALYREF/THOC4 is reported conflictingly with [PubMed:23299939] reporting a stimulatory effect. [UniProtKB/Swiss-Prot Function]
Synonyms	BAT1; D6S81E; UAP56
Reference Data
Protein Pathways	Spliceosome

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