ARHGEF4 Mouse Monoclonal Antibody [Clone ID: OTI1E5]

Specifications

Product Data
Clone Name	OTI1E5
Applications	WB
Recommend Dilution	WB 1:500
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-363 of human ARHGEF4 (NP_056135) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
Predicted Protein Size	78.9 kDa
Gene Name	Rho guanine nucleotide exchange factor 4
Database Link	NP_056135 Entrez Gene 50649 Human UniProt ID Q9NR80
Background	Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jun 2013].
Synonyms	ASEF; ASEF1; GEF4; SMIM39; STM6
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Regulation of actin cytoskeleton

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