Lysyl tRNA synthetase (KARS) Mouse Monoclonal Antibody [Clone ID: OTI1E7]

Specifications

Product Data
Clone Name	OTI1E7
Applications	IHC, WB
Recommend Dilution	WB 1:500, IHC 1:500
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human KARS (NP_005539) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	67.9 kDa
Gene Name	lysyl-tRNA synthetase 1
Database Link	NP_005539 Entrez Gene 85305 MouseEntrez Gene 292028 RatEntrez Gene 3735 Human UniProt ID Q15046
Background	Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Synonyms	CMTRIB; DEAPLE; DFNB89; KARS; KARS2; KRS; LEPID
Reference Data
Protein Pathways	Aminoacyl-tRNA biosynthesis

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