Nucleostemin (GNL3) Mouse Monoclonal Antibody [Clone ID: OTI4E1]

Specifications

Product Data
Clone Name	OTI4E1
Applications	WB
Recommend Dilution	WB 1:500
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human GNL3 (NP_055181) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	61.8 kDa
Gene Name	G protein nucleolar 3
Database Link	NP_055181 Entrez Gene 26354 Human UniProt ID Q9BVP2
Background	The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Synonyms	C77032; E2IG3; NNP47; NS
Reference Data
Protein Families	ES Cell Differentiation/IPS, Stem cell - Pluripotency

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