Cd4 Rat Monoclonal Antibody [Clone ID: CT-CD4]

Specifications

Product Data
Clone Name	CT-CD4
Applications	FC
Recommend Dilution	Flow Cytometry.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	This antibody CL004F recognizes CD4 . Results for tissue distribution by Flow Cytometry analysis (Mouse strain BALB/c): Cell source Spleen T cells: Percentage of cells stained above control = 59.2% Cell source Thymus: Percentage of cells stained above control = 93.4% (Cell concentration = 1x106 cells per test, antibody concentration = 1.0 µg/106 cells, isotypic control FITC Rat IgG2a).
Formulation	PBS Label: FITC State: Liquid purified Ig fraction Stabilizer: EIA grade BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	CD4 antigen
Database Link	Entrez Gene 12504 Mouse UniProt ID P06332
Background	CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Synonyms	T-cell surface antigen T4/Leu-3
Note	Protocol: FLOW CYTOMETRY ANALYSIS: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population . 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x106 cells, representing 1 test). 4. To each tube, add ~1.0 µg of CL004F per 1x106 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes be protected from light, since most fluorochromes are light sensitive.) 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + Sodium Azide (100 µl of 2M Sodium Azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine Serum Albumin + Sodium Azide (100 µl of 2M sodium azide in 100 mls). Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: C57BL/6 Cell Concentration: 1x106 cells per test Antibody Concentration Used: 1.0 µg/106 cells Isotypic Control: FITC Rat IgG2a
Reference Data

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