Cd8a Rat Monoclonal Antibody [Clone ID: YTS169.4]

CAT#: CL007F

Cd8a rat monoclonal antibody, clone YTS169.4, FITC



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CNY 3,740.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name YTS169.4
Applications FC, IHC
Recommend Dilution Flow Cytometry.
Immunohistochemistry on frozen sections.
Reactivity Mouse
Host Rat
Clonality Monoclonal
Immunogen Murine thymocytes
Specificity Antibody CL007 reacts with a protein of approximately 30 kDa found on mouse thymocytes and mouse cytotoxic/ suppressor T cells. It does not bind to mouse helper/inducer T cells. It binds to T lymphocytes from all mouse strains regardless of phenotypic expression (i.e. reacts with T lymphocytes from mouse strains expressing the Ly 2.1 or Ly 2.2 phenotype). It can be used to investigate the role of T cells in models for infectious disease, autoimmunity, transplantation tolerance and fundamental aspects of immunology.
Formulation PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Label: FITC
State: Liquid purified IgG
Concentration lot specific
Purification Protein G Chromatography
Conjugation FITC
Storage Condition Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name CD8 antigen, alpha chain
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
Synonyms CD8 alpha chain, CD8A, MAL
Note Strain Distribution by Flow Cytometry Analysis:
Procedure: see below
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 0.1 µg/10e6 cells
Strains Tested: BALB/c, C57BL/6
Positive: BALB/c, C57BL/6
Negative: non

Protocol: FLOW CYTOMETRY ANALYSIS:
Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50µl of this suspension to each tube (each tube will then contain 1 x 106 cells, representing 1 test).
4. To each tube, add 0.1-0.5 µg* of CL007F per 106 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
(It is recommended that the tubes are protected from light, since most flurochromes are light sensitive.)
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 µl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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