T Cell Receptor (TCR) alpha/beta Hamster Monoclonal Antibody [Clone ID: H57-597]

Specifications

Product Data
Clone Name	H57-597
Applications	FC
Recommend Dilution	Use of this antibody in conjunction with an anti-CD3e monoclonal antibody (Cat.-No CL001F or CL001FX) allows for accurate measurements of the mutually exclusive sub-populations of alpha/beta TCR and gamma/theta TCR bearing T cells.
Reactivity	Mouse
Host	Hamster
Clonality	Monoclonal
Immunogen	Affinity-purified DO-11.10 TCR from Armenian Hamster
Specificity	Mouse alpha/beta T Cell Receptor. This monoclonal antibody reacts with the surface of all alpha/beta TCR bearing cells and does not react with receptors on gamma/theta TCR positive T cells. This monoclonal antibody when used in an immobilized form was able to activate all alpha/beta TCR bearing T cell hybridomas tested to produce IL-2 (1).
Formulation	PBS with 0.02% Sodium azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. Label: PE State: Liquid purified IgG fraction. Label: R-Phycoerythrin
Concentration	lot specific
Purification	Protein G Chromatography.
Conjugation	PE
Storage Condition	Store the antibody at 2-8°C. DO NOT FREEZE! Avoid prolonged exposure to light.
Synonyms	TCRA, TCRB, T-Cell Receptor alpha, T-Cell Receptor beta, T-Cell Receptor alpha beta
Note	Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with IQ-Lyse (IQP-199) or similar cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 1.0-0.5 µg* of CL075R or CL075RX per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.) 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). Results-Tissue Distribution: Mouse Strain: BALB/C Cell Concentration: 1x10e6 cells per test. Antibody Concentration Used: 1.0 µg/10e6 cells. Isotypic Control: PE Hamster IgG. Cell Source: A/ Splenic T cells B/ Thymus Percentage of Cells Stained Above Control: A /89.3 B /75.2 N.B. Appropriate control samples should always be included in any labelling studies. *For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use. STRAIN DISTRIBUTION: Antibody Concentration: 1.0 ug/10e6 cells Strains Tested: C57BL/6, CBA/J, AKR, BALB/c, C3H/He Postive: CBA/J, BALB/c, C3H/He, AKR, C57BL/6 Negative: None.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.