BETVIA Human Monoclonal Antibody [Clone ID: H.G5F2A4.09]

Specifications

Product Data
Clone Name	H.G5F2A4.09
Applications	ELISA, WB
Recommend Dilution	ELISA, histamine release blocking , WB Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml.
Reactivity	Pollen
Host	Human
Clonality	Monoclonal
Immunogen	human B cells immortalized from a birch pollen allergic patient
Specificity	birch pollen Bet v1 Cross-reactivity: large panel of tree’s pollen
Formulation	State: Purified State: 100 μg in 200 μl Tris-NaCl pH 8
Concentration	lot specific
Purification	QMA Hyper-D Ion-exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Background	Birch pollen allergy is a frequent pathology with IgE reactivity against Betv1 the major birch pollen allergen. Human monoclonal antibodies secreting B cells have been cloned from PBMC of allergic patients. These high affinity human monoclonal antibodies raised against BetV1 inhibit the binding of IgE to nitrocellulose-blotted Bet v1 and inhibit histamine release by sensitized basophils. (Visco V. et al, J. Immunol., 1996 ; 157: 956-962 ; Denépoux S et al, FEBS Lett. 2000 Jan 7;465(1):39-46.)
Synonyms	BETVI, Allergen Bet v I-A
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.