CD40L (CD40LG) Mouse Monoclonal Antibody [Clone ID: LL2]

Specifications

Product Data
Clone Name	LL2
Applications	ELISA, FC, FN, IF
Recommend Dilution	Flow cytometry, IF, T cell activation, CD40-CD40L blocking, ELISA for sCD154 detection. *This monoclonal antibody may be used between 5-20 μg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Chimeric Human CD40L-Ig-transfected COS-7 cells.
Specificity	human CD40L.
Formulation	100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8 State: Purified
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	CD40 ligand
Database Link	Entrez Gene 959 Human UniProt ID P29965
Background	CD40 ligand also known as TRAP, gp39, TNFSF5, is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF superfamily. CD40 ligand is expressed predominantly on activated CD4+ T cells and is also found on other cell types, including NK cells, mast cells, basophils and eosinophils. Genetic alterations in the CD40L results in the absence of a functional molecule on activated T cells and are responsible for the occurrence of X-linked hyper-IgM syndrome. LL2 clone was produced by immunizing mice with L cells transfected with human CD40L. Addition of anti-CD40L mAb LL2 enhanced proliferation of MT9 and FS35 T cell lines in the presence of IL-2 and increased the secretion of IFN-γ by MT9 cells and of IL-10, IL-5 and IL-4 by FS35 cells cultured with IL-2. LL2 completely blocks CD40- CD40L interaction, can mimic T cell-induced CD40 triggering on B cells and activates T cells. (Hermann P et al, 1995, EJI, 25 (10), 2972-2977; Razanajaona D et al, 1996, J Immunol., 157(4):1492-8 ; Fournier N et al, 2000 J Immunol., 165: 1197–1209; Henn et al, 2001, Blood, 98 (4), 1047-1054).
Synonyms	CD40 ligand, CD40-L, CD40LG, TNFSF5, TRAP, GP39
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.