DC SIGN (CD209) (Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: 128G8.43]

Specifications

Product Data
Clone Name	128G8.43
Applications	FC, IHC, WB
Recommend Dilution	Antigen uptake, FACS surface, ImmunoHistoChemistry frozen sections, Western Blot.
Reactivity	Human, Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	human (GMCSF + TNF) DC subset.
Specificity	Human (epitope in extracellular domain). Species cross-reactivity: Sheep.
Formulation	Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8 State: Purified
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	CD209 molecule
Database Link	Entrez Gene 30835 Human UniProt ID Q9NNX6
Background	We have generated an antibody recognizing an antigen which has a tissular distribution and a size (western blot) identical to DC-SIGN. This antibody does not recognize DC-SIGN 1 expressed in HeLa cells. DCSIGN (“DC Specific, ICAM-3 Grabbing, Nonintegrin”) / CD209 is a type II membrane protein with an external mannose-binding C-type lectin domain, DC-SIGN is expressed by immature and mature dendritic cells (DC). In the skin, DC-SIGN+ DC are exclusively located in the dermis. DC-SIGN binds to ICAM-3 on resting T cells, establishing DC-T cell contact and adaptive immunity. DC-SIGN is a high affinity receptor for HIV gp120, allowing HIV capture and transmission to CD4+ T cells. In addition to HIV, DC-SIGN is a receptor for a number of other viral and cellular pathogens including Mycobacterium Tuberculosis, and is a major player in microbial evasion of the immune system. (Geijtenbeek, T and al, Cell; 2000; 100: 587-597; van Kooyk Y and al, Nat. Rev. Immunol.; 2003; 3: 697- 709)
Synonyms	DCSIGN1, DCSIGN, DC-SIGN1, CLEC4L, Dendritic Cell Marker
Reference Data

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