DC SIGN (CD209) Mouse Monoclonal Antibody [Clone ID: 111H2.02]

CAT#: DDX0209A488-100

DC SIGN (CD209) mouse monoclonal antibody, clone 111H2.02



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CNY 6,281.00


货期*
4周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 111H2.02
Applications FC
Recommend Dilution DDX0209P-50 / DDX0209P-100 purified: Surface flow cytometry, IHC Formol- paraffin, Bouin paraffin, Histowax.
DDX0209A488-50 / DDX0209A488-100 Alexa-Fluor®488: Surface Flow cytometry.
DDX0209A546-50 / DDX0209A546-100 Alexa-Fluor®647: IF. 
DDX0209A647-50 / DDX0209A647-100 Alexa-Fluor®647:
Surface Flow cytometry. 
DDX0209B-50 DDX0209B-100 Biotin (on request): Surface Flow cytometry, IHC. 
Usage recommendation:
*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen HeLa cells stably transfected-with human DC-SIGN.
Specificity Human DC-SIGN.
Species cross-reactivity: positive staining on NIH3T3-transfected LSIGN.
Formulation Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
Label: Alexa Fluor 488
Concentration lot specific
Conjugation Alexa Fluor 488
Storage Condition -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Gene Name CD209 molecule
Background DC-SIGN (“DC Specific, ICAM-3 Grabbing, Nonintegrin”)/CD209 and liver/lymph node-specific ICAM-3-grabbing nonintegrin (L-SIGN) (CD299/DC-SIGNR for DC-SIGN-related molecule; DC-SIGN2) are closely related genes that map to chromosome 19p13.3.Both genes encode a member of the C-type lectin family of type II transmembrane proteins. The two receptors are 77% identical at the amino acid level, have similar ligands. They are expressed in different tissues. Both receptors have been shown to interact with ICAM-3 DC-SIGN is a high affinity receptor for HIV gp120 (Soilleux EJ. 2003, Clinical Science 104, 437-; Dakappagari N., et al. 2006, The J Immunol, 176, 426; Geijtenbeeck T.B., et al. 2000, Cell, 100, 57 ; .Bashirova A. et al., 2001, J.Exp. Med., 193, 671). The clone 111H2.02 anti-DC-SIGN blocks HIVgp120-DC-SIGN interaction and reacts on formalin-fixed tissues (Canard B et al, Immunol Lett. 2011, 135(1-2):165-72).
Synonyms DCSIGN1, DCSIGN, DC-SIGN1, CLEC4L, Dendritic Cell Marker
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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