Peptidase 1 / DERP1 Human Monoclonal Antibody [Clone ID: H.AK5.C]

Specifications

Product Data
Clone Name	H.AK5.C
Applications	ELISA, WB
Recommend Dilution	ELISA, Western Blot, Biopanning.  Usage recommendation: *This monoclonal antibody may be used between 1-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use.
Reactivity	Human
Host	Human
Clonality	Monoclonal
Immunogen	EBV cell clones derived from PBMC of allergic patients to DPT.
Specificity	allergen of the house-dust mites Dermatophagoides pteronyssinus Derp 1
Formulation	100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8 State: Purified
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Background	House-dust mite allergy belongs to the most common allergies worldwide which affect more than 50% of all allergic patients. House dust mite allergens play an important role in inducing IgE-mediated sensitization and the development of bronchial hyperresponsiveness and asthma, perennial rhinitis, and atopic dermatitis. Allergic patients’s sera (IgE) recognize the house-dust mite Dermatophagoides Pteronyssinus Derp 1. The key event in these reactions is the cross-linking of IgE antibodies on effector cells by allergens, which induces a release of biologically active mediators like histamine and leukotrienes leading to different symptoms of allergy. Studies in vivo have shown that the cysteine peptidase activity of group 1 house dust mite allergens contributes to their allergenicity. (Chapman MD, 1980; J. Immunol., 125: 587-592; Hales BJ, Thomas WR., 1997; Clin. Exp. Allergy, 27(8):868- 875; Doull IJ. et al, 1997; Allergy, 52,220; Custovic A, 1996; J. Allergy Clin. Immunol., 98: 64- 72; Szalai K. et al., 2008; Mol. Immunol. 45:5, 1308-17)
Synonyms	Allergen Der p I
Reference Data

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