Influenza M1/HLA-A2 Complex (Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: 405H1.01]

Specifications

Product Data
Clone Name	405H1.01
Applications	FC
Recommend Dilution	DDX0270P-50 / DDX0270P-100 Purified: Flow cytometry, ELISA. DDX0270A488-50 / DDX0270A488-100 Alexa-fluor®488: Flow cytometry. DDX0270A546-50 / DDX0270A546-100 Alexa-fluor®488: Flow cytometry.. DDX0270A647-50 / DDX0270A647-100 Alexa-fluor®488: Flow cytometry. DDX0270B-50 / DDX0270B-100 Biotin: ELISA. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	T2 cell line charged in M1 peptide
Specificity	Human extracellular/M1 HLA neuropilin-1(epitope in extracellular domain).
Formulation	Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol. Label: Alexa Fluor 488
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Alexa Fluor 488
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Background	In order to generate this mAb, the HLA.A2 heavy chain and human 2-m were produced in bacteria, purified and covalently bound to the immunodominant HLA.A2 restricted peptide 58-66 of the influenza virus matrix protein (M1p). Correct refolding was confirmed by staining specific CD8+ CTL clones with the biotinylated recombinant chimera that had been tetramerized with PE-streptavidin. Balb/c mice were immunized with the chimera and hybridomas were tested by a differential chimera-based ELISA containing M1-HLA.A2 versus HIVgag-HLA.A2 and empty HLA.A2 constructs. 405H1 mAb was found to be specific for M1-HLA.A2 with a Kd = 3,3.10-10 M. 405H1 represents a valuable tool for studying the processing and direct or cross-presentation on MHC-I molecule.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.