MMP12 Mouse Monoclonal Antibody [Clone ID: 603E6.22]

Specifications

Product Data
Clone Name	603E6.22
Applications	IHC
Recommend Dilution	DDX0280P-50 / DDX0280P-100 Purified: ELISA, ImmunoHistoChemistry frozen sections, Immunoprecipitation.  DDX0280B-50 / DDX0280B-100 Purified: ImmunoHistoChemistry frozen sections.  Usage recommendation: *This monoclonal antibody may be used between 5-20μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	recombinant HME
Specificity	Human HME-MMP12 (epitope in proenzymatic region).
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol. Label: HRP
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	HRP
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	matrix metallopeptidase 12
Database Link	Entrez Gene 4321 Human UniProt ID P39900
Background	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. HME/MMP-12, also called metalloelastase, is reported only in few cells, including tissue macrophages and hypertrophic chondrocytes. MMP-12 is critical for invasion and destruction in pathologies such as aneurysm and emphysema. The predicted molecular mass of the HME proenzyme is 54 kDa. HME mRNA and protein were detected in human alveolar macrophages. Similar to murine macrophage metalloelastase, HME readily undergoes NH2- and COOH-terminal processing to a mature 22 kDa form. Both recombinant expressed in Escherichia Coli and native HME derived from human alveolar macrophage-conditioned media degraded insoluble elastin. HME is a unique human metalloproteinase that displays elastolytic activity and is expressed in alveolar macrophages; MMP12 mediates smoke-induced inflammation by releasing TNFa from macrophages, with subsequent endothelial activation, neutrophil influx, and proteolytic matrix breakdown caused by neutrophil-derived proteases. (Demets IK et al, 2006; Thorax, 61:196-201)
Synonyms	Macrophage metalloelastase, HME, ME, Matrix metalloproteinase-12, MMP12, Macrophage elastase
Reference Data

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