IL3RA Mouse Monoclonal Antibody [Clone ID: 107D2.08]

Specifications

Product Data
Clone Name	107D2.08
Applications	FC, IF
Recommend Dilution	DDX0300P-50 DDX0300P-100 Purified: FACS surface, ImmunoHistoChemistry frozen sections, ImmunoHistoChemistry paraffin sections, Immunoprecipitation. DDX0300A488-50 DDX0300A488-100 AlexaFluor®488: FACS surface, Immunofluorescence. DDX0300A546-50 DDX0300A546-100 AlexaFluor®546: Immunofluorescence. DDX0300A647-50 DDX0300A647-100 AlexaFluor®647: Surface and Intracyto Flow cytometry DDX0300B-50 DDX0300B-100 Biotin: Immunoprecipitation, ImmunoHistoChemistry paraffin sections, ImmunoHistoChemistry frozen sections. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use.
Reactivity	Canine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	sorted human tonsillar pDC
Specificity	Human CD123 (IL3Ra). Species cross-reactivity: Dog.
Formulation	Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol. Label: Alexa Fluor 488
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Alexa Fluor 488
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	interleukin 3 receptor subunit alpha
Database Link	Entrez Gene 3563 Human UniProt ID P26951
Background	IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The α subunit (CD123) specifically binds IL3, whereas the b subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment. (Bendriss-Vermare N thesis, 2001;Treilleux I et al, 2004 ; Clin. Canc. Res., 10: 7466-7474).
Synonyms	Interleukin-3 receptor subunit alpha, IL-3R-alpha, IL3 receptor alpha
Reference Data

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