Langerin (CD207) Mouse Monoclonal Antibody [Clone ID: DCGM4/122D5.03]

Specifications

Product Data
Clone Name	DCGM4/122D5.03
Applications	FC, IF
Recommend Dilution	DDX0363P-50 DDX0363P-100 Purified: Internalization, Flow cytometry, IHC. DDX0363A488-50 DDX0363A488-100 Alexa-fluor®488: Flow cytometry, IF. DDX0363A546-50 DDX0363A546-100 Alexa-fluor®546: IF. DDX0363A647-50 DDX0363A647-100 Alexa- fluor®647: Flow cytometry. DDX0363B-50 DDX0363B-100 Biotin: Western blot. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined y each laboratory for each application. *Coupled antibody: to maintain RT before using.
Reactivity	Canine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	In vitro-derived human DCs from CD34+ cells in GM-CSF+TNFα
Specificity	Human Langerin (extracellular epitope in Carbohydrate Recognition domain). Species cross-reactivity: Dog.
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol. Label: Alexa Fluor 488
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Alexa Fluor 488
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	CD207 molecule
Database Link	Entrez Gene 50489 Human UniProt ID Q9UJ71
Background	Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck’s granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 ; Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)
Synonyms	CLEC4K, Dendritic Cell Marker
Reference Data

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