Mannose Receptor (MRC1) (Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: 122D2.08]

Specifications

Product Data
Clone Name	122D2.08
Applications	FC, FN, IHC, IP, Neutralize, WB
Recommend Dilution	Surface Flow Cytometry, WB, IHC, IP, internalization, inhibition of antibody binding by mannan, inhibition of dextran-fitc uptake by mannose-R expressing cells
Reactivity	Human, Porcine, Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	human (GM+TNF) DC subset
Specificity	human mannose receptor (extracellular part). Species cross-reactivity: swine, sheep
Formulation	Purified: Tris-NaCl pH 8. Coupled: PBS 50% glycerol (on request). State: Purified
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	mannose receptor, C type 1
Database Link	Entrez Gene 4360 Human UniProt ID P22897
Background	The mannose receptor (MR) /CD206 is a 175 kDa C-type lectin of type I, with 8 carbohydrate recognition domains (CRD) belonging to the pattern recognition receptors. It is an endocytic receptor of macrophages and endothelial cell subsets whose natural ligands include both self glycoproteins and microbial glycans. CD206 is predominantly expressed in tissue macrophages, myeloid dendritic cells, liver, skin, lymphoid organ and tumor. In humans, the MMR has been detected in cells located within the dermis, lamina propria, and T cell areas of the tonsil, in inflammatory epidermal DCs of atopic dermatitis patients, and in cells lining venous sinuses in the spleen. CD206 is also expressed by human CD14+ -derived DCs. mAb 122D2.08 was obtained after mice immunization with human dendritic cells. It recognizes large proteins (160-200kDa), and its binding to in vitro-generated DC is inhibited by cell preincubation with mannan, suggesting that 122D.08 is directed against the macrophage mannose receptor. The uptake of mannosylated proteins (expressed on bacteria and fungi) is important for the biologic function of DC. mAb 122D2.08 positively stains swine and sheep tissue sections (Schwartz-Cornil I, pers.communication) (Sallusto F. et al., 1995, J. Exp. Med., 182, 389-40; Wollenberg A. et al.,J Invest Dermatol, 2002, 119, 5, 1096-102 ; Marquet F et al, PLoS ONE, vol 6 (1) e16320).
Synonyms	Macrophage mannose receptor, CLEC13D, CLEC13DL, MRC1L1
Reference Data

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