Mannose Receptor (MRC1) (Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: 118F2.02]

Specifications

Product Data
Clone Name	118F2.02
Applications	FC, FN, IHC, IP, Neutralize, WB
Recommend Dilution	DDX0381P-50 / DDX0381P-100 purified: Surface Flow Cytometry, WB, IHC, IP, internalization, inhibition of antibody binding by mannan, dextran-FITC uptake.
Reactivity	Human, Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	Human CD34+ (GM+TNF)-derived DC.
Specificity	Human mannose receptor (extracellular part).- Species cross-reactivity: Sheep.
Formulation	Purified: 100 μg in 200 μl Tris-NaCl pH 8. Coupled: 100 μg in 200 μl PBS 50% glycerol. State: Purified
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	mannose receptor, C type 1
Database Link	Entrez Gene 4360 Human UniProt ID P22897
Background	The mannose receptor (MR) /CD206 is a 175 kDa C-type lectin of type I membrane molecule, with 8 carbohydrate recognition domains (CRD) belonging to the pattern recognition receptors. It is an endocytic receptor of macrophages and endothelial cell subsets whose natural ligands include both self glycoproteins and microbial glycans. CD206 is predominantly expressed in tissue macrophages, myeloid dendritic cells, liver, skin, lymphoid organ and tumor. In humans, the MMR has been detected in cells located within the dermis, lamina propria, and T cell areas of the tonsil, in inflammatory epidermal DCs of atopic dermatitis patients, and in cells lining venous sinuses in the spleen. CD206 is also expressed by human monocyte-derived DCs. mAb 118F2.02 was obtained after mice immunization with human dendritic cells. It recognizes large proteins (160- 200kDa), and its binding to in vitro-generated DC is inhibited by cell preincubation with mannan, suggesting that 118F2.02 is directed against the macrophage mannose receptor. The uptake of mannosylated proteins (expressed on bacteria and fungi) is important for the biologic function of DC. (Sallusto F. et al., 1995, J. Exp. Med., 182, 389-40; Wollenberg A. et al.,J Invest Dermatol, 2002, 119, 5, 1096-102)
Synonyms	Macrophage mannose receptor, CLEC13D, CLEC13DL, MRC1L1
Reference Data

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