MIP3 alpha / CCL20 Mouse Monoclonal Antibody [Clone ID: 206D9.05]

Specifications

Product Data
Clone Name	206D9.05
Applications	FN, IHC, WB
Recommend Dilution	DDX0420P-50 DDX0420P-100 Purified: Blocking cell migration, ImmunoHistoChemistry frozen sections, ImmunoHistoChemistry paraffin sections, Western Blot. DDX0420HRPO-50 DDX0420HRPO-100 HRPO: ELISA detection (with 319F6.06 clone). Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before using.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant MIP3α in eukaryotic cells.
Specificity	Human MIP-3α.
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol. State: Purified
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	Aliquot storage conditions -20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Background	Macrophage inflammatory protein (MIP)-3alpha/ CCL20 is a CC-type chemokine mapped to chromosome 2 in humans. Langerhans cells (LCs) represent a unique population of DCs colonizing epithelium. MIP-3alpha plays a central role in LC precursor recruitment into the epithelium during inflammation. (a) Among DC populations, MIP-3alpha is the most potent chemokine inducing the selective migration of in vitrogenerated CD34(+) hematopoietic progenitor cell-derived LC precursors and skin LCs in accordance with the restricted MIP-3alpha receptor (CC chemokine receptor 6) expression by these cells. (b) MIP-3alpha is mainly produced by epithelial cells, and the migration of LC precursors induced by the supernatant of activated skin keratinocytes is blocked with an antibody against MIP-3alpha. (c) In vivo, MIP-3alpha is selectively produced at sites of inflammation as illustrated in tonsils and lesional psoriatic skin where MIP- 3alpha upregulation appear associated with an increase in LC turnover. (d) The secretion of MIP-3alpha is strongly upregulated by cells of epithelial origin after inflammatory stimuli (interleukin 1beta plus tumor necrosis factor alpha) or T cell signals.(Dieu-Nosjean et al, J;Exp.med, 2000 192: 705-18;Homey B. et al, 2000,J.Immunol.164:6621-32).
Synonyms	CCL20, LARC, MIP3A, MIP-3 alpha, C-C motif chemokine 20, Small-inducible cytokine A20, SCYA20, Exodus-1, Dendritic Cell Marker, DC marker
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.